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Mouse EGF / Epidermal Growth Factor Protein (Fc Tag)

Mouse EGF / Epidermal Growth Factor Protein (Fc Tag)

产品编号:G1008
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Sample solution is provided at 25 µL, 10mM.

Description

Synonym AI790464
Protein Construction A DNA sequence encoding the mature form of mouse EGF ( P01132 ) ( Asn 977 - Arg 1029 ) was fused with the Fc region of human IgG1 at the N-terminus
Source Mouse
Expression Host Human Cells
Purity (67.3+30.0) % as determined by SDS-PAGE
Bio-activity Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
The ED50 for this effect is typically 0.2-1 ng/mL.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Stability Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal Glu
Molecular Mass The recombinant mouse EGF/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 314 amino acids and has a predicted molecular mass of 34.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 44 and 38 kDa.
Formulation Lyophilized from sterile PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Storage Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
MSTN / GDF8 Protein Description

Mouse? epidermal growth factor ( EGF ), also known as urogastrone (URG) since it was isolated from urine based on its inhibitory effect on gastric secretion, is the founding member of the EGF-family of growth factors. All the members of this protein family have highly similar structural and functional characteristics. EGF is initially synthesized as a 130 kDa single-pass transmembrane protein containing 9 EGF-like units and 9 LDL-receptor class B repeats. The mature soluble EGF is a 6 kDa peptide corresponding to the EGF unit located proximal to the transmembrane domain. EGF is expressed in kidney, salivary gland, cerebrum and prostate, and plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. As a potent mitogen, EGF stimulates the growth of various epidermal and epithelial tissues both in vivo and in vitro. Furthermore, EGF is also identified as a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4), a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion.