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Mouse SCF / C-kit ligand Protein (His Tag)

Mouse SCF / C-kit ligand Protein (His Tag)

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Sample solution is provided at 25 µL, 10mM.

Description

Synonym

Clo,Con,Gb,Kitlg,Mgf,SCF,SF,SLF,Sl,Steel,c ontrasted

Protein Construction

A DNA sequence encoding the amino acids ( Met 1 - Ala 189 ) of mouse KITL ( P20826-1 ) was fused with a polyhistidine tag at the C-terminus

Source Mouse
Expression Host Baculovirus-Insect cells
Purity > 95 % as determined by SDS-PAGE
Bio-activity

1. Measured by its binding ability in a functional ELISA
Immobilized mouse KITL at 2 μg/ml ( 100 μl/well ) can bind mouse CD117 with a linear ranger of 1.28 - 32 ng/ml

2. Measured in a cell proliferation assay using?TF-1?human erythroleukemic cells ( Kitamura,T. et?al.?1989, J.?Cell?Physiol.?140:323. )
The EC50?for this effect is typically? 0.5 - 5 ng / ml
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Stability Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal Lys 26
Molecular Mass

The secreted recombinant mouse KITL consists of 175 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions

Formulation Lyophilized from sterile 20 mM Tris , 50 mM NaCl, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Storage Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
KITL / Kitlg ( aa 1 - 189 ) Protein Description
Mouse kit ligand, also known as Hematopoietic growth factor KL, Mast cell growth factor, Steel factor, Stem cell factor, c-Kit ligand, Kitlg and KITL, is a single-pass type I membrane protein which belongs to the?SCF family. KITL / kit ligand also belongs to the family of dimeric transmembrane growth factors. The soluble form of KIT ligand is a secreted protein. Mast cells are thought to participate in a variety of immune responses, such as parasite resistance and the allergic reaction. Mast cell development depends on stem cell factor (Kit ligand) and its receptor, c-Kit. KITL / kit ligand stimulates the proliferation of mast cells. KITL / kit ligand is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Efficient cell surface presentation of KITL / kit ligand is essential for the migration, proliferation, and survival of melanocytes, germ cells, hemopoietic stem cells, and mastocytes. KITL / kit ligand acts synergistically with other cytokines, probably interleukins. KITL / kit ligand plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. It exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes. KITL / kit ligand misexpression in some hyperpigmented lesions may open the avenue for Kitl-dependent treatment of pathological skin conditions